《植物生理学报》 2017, 53(8): 1435-1443

ACS2参与拟南芥幼苗根生长响应低磷反应的机制

吕淑芳^1,2, 彭媛¹, 江静^1,*

¹河南大学生命科学学院, 棉花生物学国家重点实验室, 植物逆境生物学重点实验室, 河南开封475004; ²河南科技大学农学院, 河南洛阳471003

通信作者：江静；E-mail: jiangjing@henu.edu.cn

摘　要：

磷元素是植物生长发育所必须的, 低磷不利于植物根生长发育。植物激素乙烯生物合成前体物质1-氨基环丙烷-1-羧酸(ACC)调节植物根生长。ACC合酶(ACS)家族成员ACS2被证明与植物根生长有关。本研究的结果显示, 在低磷抑制拟南芥幼苗根生长过程中, 根部ACS2表达量和ACC含量增加, 但无机磷含量降低。其降低机制可能是ACC降低了磷吸收和运输因子PHO1活性, 因此改变了无机磷在根中的积累和再分配。因此推测, ACS2表达是低磷抑制拟南芥幼苗根生长的重要环节。

关键词：ACS2; ACC; 低磷; 根生长

收稿：2017-02-20   修定：2017-07-06

资助：国家自然科学基金(31271510)。

The involvement of ACS2 in low phosphate-mediated root growth of Arabidopsis seedling

LÜ Shu-Fang^1,2, PENG Yuan¹, JIANG Jing^1,*

¹State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng, Henan 475004, China; ²College of Agricultural, Henan University of Science and Technology, Luoyang, Henan 471003, China

Corresponding author: JIANG Jing; E-mail: jiangjing@henu.edu.cn

Abstract:

Phosphorus is essential for plant growth and development, and low phosphate (Pi) changes the plant vital process and morphology. The ethylene biosynthesis precursor 1-aminocyclopropane-1-carboxylate acid (ACC) regulated the root growth of plant, and ACC synthase (ACS) family member ACS2 was also proved to be related with plant root growth. In this paper, we found that overexpression of ACS2 gene significantly decreased the root length under low Pi, compared with wild type (WT). Further study results showed that the root growth were inhibited in Arabidopsis seedlings, when the expression of ACS2 increased and the content of ACC in roots raised. Whereas the content of Pi decreased significantly, especially in roots. These phenomenons might indicate that the expression of phosphate uptake and transport factor PHO1 were inhibited by ACC, which reduced and changed the Pi accumulation and redistribution in root under low Pi. These results, together with above findings, suggested that the inhibition of Arabidopsis thaliana root growth maybe dependent on the expression of ACS2 under low phosphate.

Key words: ACS2; ACC; low phosphate; root growth